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Metabolic engineering of Escherichia coli for microbial production of L-methionine.
- Source :
- Biotechnology & Bioengineering; Apr2017, Vol. 114 Issue 4, p843-851, 9p
- Publication Year :
- 2017
-
Abstract
- ABSTRACT L-methionine has attracted a great deal of attention for its nutritional, pharmaceutical, and clinical applications. In this study, Escherichia coli W3110 was engineered via deletion of a negative transcriptional regulator MetJ and over-expression of homoserine O-succinyltransferase MetA together with efflux transporter YjeH, resulting in L-methionine overproduction which is up to 413.16 mg/L. The partial inactivation of the L-methionine import system MetD via disruption of metI made the engineered E. coli Δ metJ Δ metI/pTrcA*H more tolerant to high L-ethionine concentration and accumulated L-methionine to a level 43.65% higher than that of E. coli W3110 Δ metJ/pTrcA*H. Furthermore, deletion of lysA, which blocks the lysine biosynthesis pathway, led to a further 8.5-fold increase in L-methionine titer of E. coli Δ metJ Δ metI Δ lysA/pTrcA*H. Finally, addition of Na<subscript>2</subscript>S<subscript>2</subscript>O<subscript>3</subscript> to the media led to an increase of fermentation titer of 11.45%. After optimization, constructed E. coli Δ metJ Δ metI Δ lysA/pTrcA*H was able to produce 9.75 g/L L-methionine with productivity of 0.20 g/L/h in a 5 L bioreactor. This novel metabolically tailored strain of E. coli provides an efficient platform for microbial production of L-methionine. Biotechnol. Bioeng. 2017;114: 843-851. © 2016 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00063592
- Volume :
- 114
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Biotechnology & Bioengineering
- Publication Type :
- Academic Journal
- Accession number :
- 121368349
- Full Text :
- https://doi.org/10.1002/bit.26198