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SATB1 promotes tumor metastasis and invasiveness in oral squamous cell carcinoma.
- Source :
- Oral Diseases; Mar2017, Vol. 23 Issue 2, p247-254, 8p
- Publication Year :
- 2017
-
Abstract
- Objective Our aim is to evaluate the expression of SATB1 in human oral squamous cell carcinomas ( OSCC) and its role in the invasiveness and metastasis of OSCC. Subjects and methods A human OSCC tissue microarray was used to evaluate the expression pattern of SATB1. SATB1 mRNA knockdown was performed in human OSCC cell lines SCC25 and Cal27 to assess the function of SATB1 in the invasiveness and metastasis of OSCC. Results SATB1 is highly expressed in human OSCC determined by immunohistochemistry, and its nuclear/cytoplasmic ratio of histoscore is significantly correlated with patients' prognosis. Reduced cell motility, invasiveness, expression of epithelial to mesenchymal transition ( EMT) markers (N-cadherin and β-catenin), and elevated expression of epithelial markers were observed in SATB1-knockdown cells in in vitro studies. Depletion of SATB1 also restored a cobblestone-like morphology in TGF-β1-treated cells. Conclusions These findings suggest SATB1 may play an important role in OSCC invasiveness and metastasis. [ABSTRACT FROM AUTHOR]
- Subjects :
- MOUTH tumors
BIOMARKERS
CANCER invasiveness
CELL lines
CELL motility
EPITHELIAL cells
GENE expression
HISTOLOGICAL techniques
IMMUNOHISTOCHEMISTRY
METASTASIS
PROBABILITY theory
PROTEINS
RESEARCH funding
SQUAMOUS cell carcinoma
SURVIVAL analysis (Biometry)
T-test (Statistics)
WESTERN immunoblotting
PROPORTIONAL hazards models
DATA analysis software
MICROARRAY technology
DESCRIPTIVE statistics
KAPLAN-Meier estimator
IN vitro studies
LOG-rank test
ONE-way analysis of variance
PROGNOSIS
Subjects
Details
- Language :
- English
- ISSN :
- 1354523X
- Volume :
- 23
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Oral Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 121199040
- Full Text :
- https://doi.org/10.1111/odi.12602