In vitro release of mitomycin C from collagen implants.

Purpose. To study Mitomycin C Loaded Collagen Implant (CI) pharmacokinetics behaviour In vitro. Methods. The CI were incubated for 15 minutes in different MMC loading solutions with the following concentrations: 0.03mg/mL (n = 9), 0.3mg/mL (n = 10) and 3.0 mg/mL (n = 10), The loaded CI were transferred in l00μL of 0.9% NaCl. Aqueous flow of 5μL/min was simulated. The MMC concentrations of the samples were determined by high performance liquid chromatography (HPLC). Dissolution kinetics were evaluated by a first-order process. The half-life of dissolution and the time of 95% dissolution were determined. Results. The Cl absorbed on average a MMC dose of 0.054, 0.530 and 6.090 μg when incubated in the different MMC loading solutions containing 0.03 mg/mL, 0.3 mg/mL. 3,0 mg/mL of MMC, respectively. In the release experiments, the mean total dose delivered by Cl was 0.0493, 0.585 and 5.291 μg. A linear correlation between loading concentration and the estimated total dose released was demonstrated. The kinetic parameters showed a fast MMC dissolution. The half-life of the 3 series was 8.8, 10.1 and 10.5 min. Conclusions. Commercially available CI can be loaded with MMC, and could provide relatively slower release than sponge delivery of MMC. Clinical implications of these results warrants further studies. [ABSTRACT FROM AUTHOR]