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Dose assessment intercomparisons within the RENEB network using G 0 -lymphocyte prematurely condensed chromosomes (PCC assay).

Authors :
Terzoudi, Georgia I.
Pantelias, Gabriel
Darroudi, Firouz
Barszczewska, Katarzyna
Buraczewska, Iwona
Depuydt, Julie
Georgieva, Dimka
Hadjidekova, Valeria
Hatzi, Vasiliki I.
Karachristou, Ioanna
Karakosta, Maria
Meschini, Roberta
M'Kacher, Radhia
Montoro, Alegria
Palitti, Fabrizio
Pantelias, Antonio
Pepe, Gaetano
Ricoul, Michelle
Sabatier, Laure
Sebastià, Natividad
Source :
International Journal of Radiation Biology; Jan2017, Vol. 93 Issue 1, p48-57, 10p
Publication Year :
2017

Abstract

Purpose: Dose assessment intercomparisons within the RENEB network were performed for triage biodosimetry analyzing G<subscript>0</subscript>-lymphocyte PCC for harmonization, standardization and optimization of the PCC assay. Materials and methods: Comparative analysis among different partners for dose assessment included shipment of PCC-slides and captured images to construct dose-response curves for up to 6 Gy γ-rays. Accident simulation exercises were performed to assess the suitability of the PCC assay by detecting speed of analysis and minimum number of cells required for categorization of potentially exposed individuals. Results: Calibration data based on Giemsa-stained fragments in excess of 46 PCC were obtained by different partners using galleries of PCC images for each dose-point. Mean values derived from all scores yielded a linear dose-response with approximately 4 excess-fragments/cell/Gy. To unify scoring criteria, exercises were carried out using coded PCC-slides and/or coded irradiated blood samples. Analysis of samples received 24 h post-exposure was successfully performed using Giemsa staining (1 excess-fragment/cell/Gy) or centromere/telomere FISH-staining for dicentrics. Conclusions: Dose assessments by RENEB partners using appropriate calibration curves were mostly in good agreement. The PCC assay is quick and reliable for whole- or partial-body triage biodosimetry by scoring excess-fragments or dicentrics in G<subscript>0</subscript>-lymphocytes. Particularly, analysis of Giemsa-stained excess PCC-fragments is simple, inexpensive and its automation could increase throughput and scoring objectivity of the PCC assay. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09553002
Volume :
93
Issue :
1
Database :
Complementary Index
Journal :
International Journal of Radiation Biology
Publication Type :
Academic Journal
Accession number :
120566297
Full Text :
https://doi.org/10.1080/09553002.2016.1234725