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Identification of pathogens by comprehensive real-time PCR versus conventional methods in community-acquired pneumonia in Japanese adults.
Identification of pathogens by comprehensive real-time PCR versus conventional methods in community-acquired pneumonia in Japanese adults.
- Source :
- Infectious Diseases; Nov/Dec2016, Vol. 48 Issue 11/12, p782-788, 7p, 3 Charts, 1 Graph
- Publication Year :
- 2016
-
Abstract
- Background:Community-acquired pneumonia (CAP) has high morbidity and mortality. Unfortunately, the pathogen detection rate using conventional culture methods is relatively low. We compared comprehensive real-time polymerase chain reaction (real-time PCR) analysis of nasopharyngeal swab specimens (NPS) and sputum samples against conventional methods for ability to detect causative pathogens of CAP. Methods:We prospectively enrolled adult CAP patients, including those with prior antibiotic use, from December 2012 to May 2014. For each patient, causative pathogens were investigated conventionally and by real-time PCR that can identify 6 bacterial and 11 viral pathogens. Results:Patients numbered 92 (mean age, 63 years; 59 male), including 30 (33%) with prior antibiotic use. Considering all patients, identification of causative pathogens by real-time PCR was significantly more frequent than by conventional methods in all patients (72% vs. 57%,p = 0.018). In patients with prior antibiotic use, identification rates also differed significantly (PCR, 77%; conventional, 50%;p = 0.027). Mixed infections were more frequent according to real-time PCR than conventional methods (26% vs. 4%,p < 0.001). By the real-time PCR,Streptococcus pneumoniaewas most frequently identified (38%) as a causative pathogen, followed byHaemophilus influenzae(37%) andMycoplasma pneumoniae(5%). PCR also identified viral pathogens (21%), with sensitivity enhanced by simultaneous examination of both NPS and sputum samples rather than only NPS samples. Conclusions:Real-time PCR of NPS and sputum samples could better identify bacterial and viral pathogens in CAP than conventional methods, both overall and in patients with prior antibiotic treatment. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 23744235
- Volume :
- 48
- Issue :
- 11/12
- Database :
- Complementary Index
- Journal :
- Infectious Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 118090755
- Full Text :
- https://doi.org/10.1080/23744235.2016.1193788