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A fluorogenic screening platform enables directed evolution of an alkyne biosynthetic tool.
- Source :
- Chemical Communications; 9/28/2016, Vol. 52 Issue 75, p11239-11242, 4p
- Publication Year :
- 2016
-
Abstract
- Directed evolution was used to improve the activity of JamB, a membrane-bound bifunctional desaturase/acetylenase. To quickly assess the protein engineering outcomes, we developed a new platform for quantifying extracellular alkyne-tagged metabolites through a fluorogenic click reaction. Random mutagenesis yielded the best JamB variant with ∼20-fold increased activity in E. coli. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13597345
- Volume :
- 52
- Issue :
- 75
- Database :
- Complementary Index
- Journal :
- Chemical Communications
- Publication Type :
- Academic Journal
- Accession number :
- 118067293
- Full Text :
- https://doi.org/10.1039/c6cc05990b