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Optimization of individualized graft composition: CD3/CD19 depletion combined with CD34 selection for haploidentical transplantation.
- Source :
- Transfusion; Sep2016, Vol. 56 Issue 9, p2336-2345, 10p, 1 Diagram, 1 Chart, 5 Graphs
- Publication Year :
- 2016
-
Abstract
- <bold>Background: </bold>Excessive T-cell depletion (TCD) is a prerequisite for graft manufacturing in haploidentical stem cell (SC) transplantation by using either CD34 selection or direct TCD such as CD3/CD19 depletion.<bold>Study Design and Methods: </bold>To optimize graft composition we compared 1) direct or indirect TCD only, 2) a combination of CD3/CD19-depleted with CD34-selected grafts, or 3) TCD twice for depletion improvement based on our 10-year experience with 320 separations in graft manufacturing and quality control.<bold>Results: </bold>SC recovery was significantly higher (85%, n = 187 vs. 73%, n = 115; p < 0.0001), but TCD was inferior (median log depletion, -3.6 vs. -5.2) for CD3/CD19 depletion compared to CD34 selection, respectively. For end products with less than -2.5 log TCD, a second depletion step led to a successful improvement in TCD. Thawing of grafts showed a high viability and recovery of SCs, but low NK-cell yield. To optimize individualized graft engineering, a calculator was developed to estimate the results of the final graft based on the content of CD34+ and CD3+ cells in the leukapheresis product.<bold>Conclusion: </bold>Finally, calculated splitting of the starting product followed by CD3/19 depletion together with CD34+ graft manipulation may enable the composition of optimized grafts with high CD34+-cell and minimal T-cell content. [ABSTRACT FROM AUTHOR]
- Subjects :
- CD34 antigen
T cells
LYMPHOCYTES
STEM cells
TRANSPLANTATION immunology
Subjects
Details
- Language :
- English
- ISSN :
- 00411132
- Volume :
- 56
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- Transfusion
- Publication Type :
- Academic Journal
- Accession number :
- 118054351
- Full Text :
- https://doi.org/10.1111/trf.13694