Effect of estradiol cypionate and Gn RH treatment on plasma estradiol-17β concentrations, synchronization of ovulation and on pregnancy rates in suckled beef cows treated with FTAI-based protocols.

Contents Two experiments were conducted to evaluate the effect of different ovulation inducers on E-17β plasma concentrations, synchronized ovulations and pregnancy rates. In Experiment 1, cows received a progesterone intravaginal device ( PID) with 1 g of progesterone (P4) plus 2 mg of estradiol benzoate ( EB) (day 0). At PID removal (day 8), cows received 0.150 mg of D-cloprostenol and were randomly assigned to four treatment groups ( n = 10/treatment): Group ECP: 1 mg of estradiol cypionate at PID removal, Group EB: 1 mg of EB 24 hr after PID removal, Group Gn RH: 10 μg of Gn RH 48 hr after PID removal, Group ECP-Gn RH: 1 mg of ECP at PID removal plus 10 μg of Gn RH 48 hr later. Ultrasonographic examinations were performed to detect the dominant follicle and ovulation. Gn RH-treated cows ovulated later ( p < .05) compared to ECP- and ECP+Gn RH-treated cows. There were effects of treatment, time and their interaction on E-17β concentrations ( p < .05). ECP treatment affected plasma E-17β concentration, which increased earlier and decreased later compared to treatments without ECP. In Experiment 2, cows received (i) ECP: n = 126; (ii) EB: n = 126; (iii) Gn RH: n = 136; (iv) ECP+Gn RH: n = 139; FTAI was performed 48-50 hr after PID removal. Pregnancy rates did not differ among ovulation inducers ( p > .05; ECP: 54.0%, 68/126; EB: 49.2%, 62/126; Gn RH: 40.4%, 55/136; ECP+Gn RH: 43.9%, 61/139). In conclusion, ECP administration ( ECP and ECP+Gn RH treatments) affected E-17β concentrations, determining its earlier increase and later decrease compared to treatments without ECP ( EB and Gn RH treatments). ECP+Gn RH-treated cows achieved the best distribution of ovulations without affecting pregnancy rates. [ABSTRACT FROM AUTHOR]