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Anti-inflammatory effects of Zea mays L husk extracts.
- Source :
- BMC Complementary & Alternative Medicine; 8/19/2016, Vol. 16, p1-8, 8p, 2 Charts, 5 Graphs
- Publication Year :
- 2016
-
Abstract
- Background: Zea mays L. (Z mays) has been used for human consumption in the various forms of meal, cooking oil, thickener in sauces and puddings, sweetener in processed food and beverage products, bio-disel. However, especially, in case of husk extract of Z mays, little is known about its anti-inflammatory effects. Therefore, in this study, the anti-inflammatory effects of Z mays husk extract (ZMHE) and its mechanisms of action were investigated. Methods: The husks of Z Mays were harvested in kangwondo, Korea. To assess the anti-inflammatory activities of ZMHE, we examined effects of ZMHE on nitric oxide (NO) production, and release of soluble intercellular adhesion molecule-1 (sICAM-1) and eotaxin-1. The expression level of inducible nitric oxide synthase (iNOS) gene was also determined by Western blot and luciferase reporter assays. To determine its mechanisms of action, a luciferase reporter assay for nuclear factor kappa B (NF-κB) and activator protein-1 (AP-1) was introduced. Results: ZMHE inhibited lipopolysaccharide (LPS)-induced production of NO in RAW264.7 cells. In addition, expression of iNOS gene was reduced, as confirmed by Western blot and luciferase reporter assays. Effects of ZMHE on the AP-1 and NF-κB promoters were examined to elucidate the mechanism of its anti-inflammatory activity. Activation of AP-1 and NF-κB promoters induced by LPS was significantly reduced by ZMHE treatment. In addition, LPS-induced production of sICAM-1 and IL-4-induced production of eotaxin-1 were all reduced by ZMHE. Conclusions: Our results indicate that ZMHE has anti-inflammatory effects by downregulating the expression of iNOS gene and its downregulation is mediated by inhibiting NF-κB and AP-1 signaling. [ABSTRACT FROM AUTHOR]
- Subjects :
- ANIMAL experimentation
ANTI-inflammatory agents
BIOLOGICAL assay
CELL adhesion molecules
CELL culture
CELLULAR signal transduction
ENZYME-linked immunosorbent assay
FLAVONOIDS
GENE expression
MICE
NITRIC oxide
OXIDOREDUCTASES
POLYPHENOLS
PROBABILITY theory
RESEARCH funding
T-test (Statistics)
TRANSCRIPTION factors
WESTERN immunoblotting
DNA-binding proteins
PLANT extracts
LIPOPOLYSACCHARIDES
DESCRIPTIVE statistics
Subjects
Details
- Language :
- English
- ISSN :
- 14726882
- Volume :
- 16
- Database :
- Complementary Index
- Journal :
- BMC Complementary & Alternative Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 117592998
- Full Text :
- https://doi.org/10.1186/s12906-016-1284-9