A high-resolution method for assessing cellular oxidative phosphorylation efficiency: bringing mitochondrial bioenergetics into focus. Focus on "Direct real-time quantification of mitochondrial oxidative phosphorylation efficiency in permeabilized skeletal muscle myofibers"

The author discusses research published within the issue which compared oxidative phosphorylation (OXPHOS) efficiency in permeabilized skeletal muscle fiber bundles (PmFBs) and isolated skeletal muscle mitochondria at lower levels of adenosine diphosphate (ADP). Topics discussed include decreased OXPHOS efficiency in PmFBs relative to isolated mitochondria, the implications of the findings, and imaging techniques that may improve resolution of measurement.