Co-immobilization of d -hydantoinase and d -carboamylase on Chitin: Application to the Synthesis of p-hydroxyphenylglycine.

The immobilization of a multi-enzyme extract is an excellent method for multi-step biotransformations. This paper describes how a multi-enzyme extract from Agrobacterium radiobacter , rich in d -hydantoinase and N-carbamyl- d -amino acid amidohydrolase was immobilized on chitin for its application on the synthesis of p-hydroxyphenylglycine. The adsorption derivative showed a higher activity than the covalent one. Compared to the soluble multi-enzymatic extract, the adsorption derivative showed greater pH-stability in the pH range under study. Its optimum pH ranged from 7-8. Furthermore, it showed high activity at low temperature. [ABSTRACT FROM AUTHOR]