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Co-immobilization of d -hydantoinase and d -carboamylase on Chitin: Application to the Synthesis of p-hydroxyphenylglycine.
- Source :
- Biocatalysis & Biotransformation; Dec2003, Vol. 21 Issue 6, p349-356, 8p
- Publication Year :
- 2003
-
Abstract
- The immobilization of a multi-enzyme extract is an excellent method for multi-step biotransformations. This paper describes how a multi-enzyme extract from Agrobacterium radiobacter , rich in d -hydantoinase and N-carbamyl- d -amino acid amidohydrolase was immobilized on chitin for its application on the synthesis of p-hydroxyphenylglycine. The adsorption derivative showed a higher activity than the covalent one. Compared to the soluble multi-enzymatic extract, the adsorption derivative showed greater pH-stability in the pH range under study. Its optimum pH ranged from 7-8. Furthermore, it showed high activity at low temperature. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10242422
- Volume :
- 21
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Biocatalysis & Biotransformation
- Publication Type :
- Academic Journal
- Accession number :
- 11715287
- Full Text :
- https://doi.org/10.1080/10242420310001597829