Biochemical and Ultrastructural Basis of the Antidiabetic Activity of Cassia fistula.

We evaluated antidiabetic efficacy of Cassia fistula stem bark extract (RG1) in 3T3-L1 cell line and streptozotocin (STZ) induced Swiss albino mice model. MTT and acute oral toxicity assays used to determined toxicity. Effect of RG1 in 3T3-L1 cell adipogenesis, glucose uptake, and pleiotropic transcriptional activators were determined in vitro antidiabetic efficacy, which was further established in vivo by using STZ induced diabetic mice model, serum biochemical analyses and the activity of RG1 in pancreatic cells. RG1 showed significant effect in 3T3-L1 adipogenesis, lipid accumulation, glucose uptake by differentiated adipocytes, and pleiotropic transcriptional activators- C/EBPa, PPARy, ap2, LPL and Adiopo-Q. RG1 affected STZ induced diabetes mice's body weight, plasma glucose level, serum lipid profile and hepatic enzyme levels as antidiabetic agent. It rejuvenated the pancreatic cell damage caused due to diabetes and restored the insulin secretory granule regeneration. The obtained results suggest RGl's mechanistic passage leading to its antidiabetic efficacy. [ABSTRACT FROM AUTHOR]