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Differential Expression of Immune Checkpoint Modulators on In Vitro Primed CD4+ and CD8+ T cells.
- Source :
- Frontiers in Immunology; 6/16/2016, p1-11, 11p
- Publication Year :
- 2016
-
Abstract
- PD-1, TIM-3, and LAG-3 are molecules shown to have immune modulatory properties, and although initially classified as indicators of T cell hyporesponsiveness, it has become clear that they are also associated with the normal course of T cell activation. Functional studies have focused mainly on CD8<superscript>+</superscript> T cells during chronic inflammation due to interest in co-opting the cellular immune response to eliminate viral or cancerous threats; however, there remains a relative lack of data regarding the expression of these molecules on CD4<superscript>+</superscript> T cells. Here, we report that expression of the immune checkpoint (IC) molecules PD-1, LAG-3, and TIM-3 are differentially expressed on CD4<superscript>+</superscript> and CD8<superscript>+</superscript> T cells in the allogeneic response resulting from a mixed lymphocyte reaction. In these studies, PD-1 expression is higher on CD4<superscript>+</superscript> T cells compared to CD8<superscript>+</superscript> T cells. In contrast, TIM-3 is expressed at higher levels on CD8<superscript>+</superscript> T cells compared to CD4<superscript>+</superscript> T cells with an apparent reciprocity in that PD-1<superscript>+</superscript> CD4<superscript>+</superscript> T cells are frequently TIM-3<superscript>lo/-</superscript>, while TIM-3-expressing CD8<superscript>+</superscript> T cells are largely PD-1<superscript>lo/-</superscript>. In addition, there is a decrease in the frequency of TIM-3<superscript>+</superscript> CD4<superscript>+</superscript> cells producing IFN-γ and IL-5 compared to TIM-3<superscript>+</superscript> CD8<superscript>+</superscript> cells. Lastly, the memory T cell phenotype within each IC-expressing subset differs between CD4<superscript>+</superscript> and CD8<superscript>+</superscript> T cells. These findings highlight key differences in IC expression patterns between CD4<superscript>+</superscript> and CD8<superscript>+</superscript> T cells and may allow for more effective therapeutic targeting of these molecules in the future. [ABSTRACT FROM AUTHOR]
- Subjects :
- T cells
CD4 antigen
IMMUNOLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 16643224
- Database :
- Complementary Index
- Journal :
- Frontiers in Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 116307120
- Full Text :
- https://doi.org/10.3389/fimmu.2016.00221