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Chemical Synthesis of K48-Linked Diubiquitin by Incorporation of a Lysine-Linked Auxiliary Handle.
- Source :
- European Journal of Organic Chemistry; May2016, p2665-2670, 6p
- Publication Year :
- 2016
-
Abstract
- We synthesized a lysine-linked auxiliary-derived building block, e-N-2-mercapto-1-(2,4-dimethoxyphenyl)ethyl lysine, in 20% isolated yield over a six-step route. The new building block is well compatible with Fmoc solid-phase peptide synthesis (SPPS) and hydrazide-based native chemical ligation (NCL). Furthermore, we successfully incorporated the new lysine-linked auxiliary handle into a protein segment and performed the chemical synthesis of K48-linked diubiquitin with stepwise NCL. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1434193X
- Database :
- Complementary Index
- Journal :
- European Journal of Organic Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 116259436
- Full Text :
- https://doi.org/10.1002/ejoc.201600292