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KOTMIN13, a Korean herbal medicine alleviates allergic inflammation in vivo and in vitro.
- Source :
- BMC Complementary & Alternative Medicine; 6/6/2016, Vol. 16, p1-10, 10p, 1 Color Photograph, 1 Chart, 4 Graphs
- Publication Year :
- 2016
-
Abstract
- Background: The ethanol extract of KOTMIN13, composed of Flowers, Semen, Inula japonica Trichosanthes kirilowii Peucedanum praeruptorum Radix, and Bulbs, was investigated for its anti-asthmatic and Allium macrostemon anti-allergic activities. Methods: The anti-asthmatic effects of KOTMIN13 were evaluated on ovalbumin (OVA)-induced murine asthma model. Anti-allergic properties of KOTMIN13 in bone-marrow derived mast cells (BMMC) and passive cutaneous anaphylaxis (PCA) in vivo were also examined. Results: In asthma model, KOTMIN13 effectively suppressed airway hyperresponsiveness induced by aerosolized methacholine when compared to the levels of OVA-induced mice. KOTMIN13 treatment reduced the total leukocytes, eosinophil percentage, and Th2 cytokines in the bronchoalveolar lavage fluids in OVA-induced mice. The increased levels of eotaxin and Th2 cytokines in the lung as well as serum IgE were decreased by KOTMIN13. The histological analysis shows that the increased inflammatory cell infiltration and mucus secretion were also reduced. In addition, the degranulation and leukotriene C4 production were inhibited in BMMC with IC<subscript>50</subscript> values of 3.9 µg/ml and 1.7 µg/ml, respectively. Furthermore, KOTMIN13 treatment attenuated mast-mediated PCA reaction. Conclusions: These results demonstrate that KOTMIN13 has anti-asthmatic and anti-allergic effects in vivo and in vitro models. [ABSTRACT FROM AUTHOR]
- Subjects :
- ASTHMA treatment
PHYTOTHERAPY
THERAPEUTIC use of plant extracts
LUNG anatomy
IMMUNOGLOBULIN analysis
ANAPHYLAXIS
ANIMAL experimentation
ASTHMA
BIOLOGICAL models
BRONCHOALVEOLAR lavage
CYTOKINES
ENZYME-linked immunosorbent assay
HIGH performance liquid chromatography
HISTOLOGICAL techniques
MAST cells
MICE
PLETHYSMOGRAPHY
PROBABILITY theory
RESEARCH funding
SPECTROPHOTOMETRY
DESCRIPTIVE statistics
LEUKOCYTE count
IN vitro studies
ONE-way analysis of variance
IN vivo studies
Subjects
Details
- Language :
- English
- ISSN :
- 14726882
- Volume :
- 16
- Database :
- Complementary Index
- Journal :
- BMC Complementary & Alternative Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 115983086
- Full Text :
- https://doi.org/10.1186/s12906-016-1155-4