STUDY OF SEMEN CHARACTERISTICS OF MURRAH BULLS DURING THE PROCESS OF FREEZING IN TWO DILUTORS (GEYT & GEYC): A COMPARATIVE STUDY.

The present study was designed to compare Glycerolated Egg Yolk Tris extender (GEYT) with Glycerolated Egg Yolk Citrate extender (GEYC) for cryopreservation of Murrah bull semen. For this purpose, ejaculates were collected from three Murrah bulls for six months by using artificial vagina at bi weekly interval. Each semen sample was divided into two parts. One part was used for the evaluation 6 of semen by conventional method, while the other part of Semen ejaculates were diluted (80 x 10 motile spermatozoa/ml) in GEYT and GEYC extender. Diluted semen was filled in straws, equilibrated for 5 hrs at 4°C; freezing was carried out in Biological freezer for 7.25 minutes and then stored in the liquid nitrogen. Thawing was performed after 24 hrs of storage, at 37°C for 45 sec. Progressive motility, live spermatozoa, abnormal spermatozoa, plasma membrane integrity and acrosomal integrity were assessed at different stages (half dilution of semen, full dilution of semen, end of equilibration and post-thawing). Amongst the two extenders used, GEYT and GEYC there was no significant difference found. [ABSTRACT FROM AUTHOR]