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Surface-engineered Saccharomyces cerevisiae cells displaying redesigned CadR for enhancement of adsorption of cadmium ( II).
- Source :
- Journal of Chemical Technology & Biotechnology; Jun2016, Vol. 91 Issue 6, p1889-1895, 7p
- Publication Year :
- 2016
-
Abstract
- BACKGROUND The use of cell-surface display technology to express recombinant proteins on a microbial cell surface can enhance metal adsorption to microbial cells. The transcription factor CadR of Pseudomonas putida shows highly selective affinity with Cd<superscript>2+</superscript>. RESULTS CadR was genetically engineered by truncating 47 N-terminal amino acids and 21 C-terminal amino acids in order to maximize its expression efficiency while maintaining its metal-binding domain. The redesigned CadR (named T68CadR) was then displayed with Saccharomyces cerevisiae a-agglutinin cell-surface display system. This engineering approach enhanced the H<superscript>+</superscript>/ OH<superscript>−</superscript> buffering capacity and the Cd<superscript>2+</superscript> adsorption capacity of yeast cells. The surface-engineered cells also performed well at mesophilic and higher temperatures (30-50 °C) under neutral or alkalescent conditions. With an initial concentration of 1.0 mg L<superscript>−1</superscript>, the Cd<superscript>2+</superscript> removal efficiency remained 85% when the concentration of Na<superscript>+</superscript> changed from 0 to 400 mmol L<superscript>−1</superscript> ( pH 7.8). The surface-engineered cells also showed highly selective adsorption to Cd<superscript>2+</superscript> in the presence of Zn<superscript>2+</superscript> and Pb<superscript>2+</superscript>. CONCLUSIONS T68CadR displayed yeast cells constructed in this study provide an option to selectively remove Cd in water. It also might be promising as a tool to determine CadR's Cd-binding mechanism and to improve its selectivity and affinity to Cd<superscript>2+</superscript>. © 2015 Society of Chemical Industry [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02682575
- Volume :
- 91
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Journal of Chemical Technology & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 115098233
- Full Text :
- https://doi.org/10.1002/jctb.4783