Characterization and Identification of Different Strains of Trichoderma Species using Bio-molecular Techniques.

Ten species of Trichoderma were isolated from the rhizospheric soil, collected from the different locations of U.P. Both morphological and molecular characterization of the isolated species was done. All the ten isolated species were screened for chitinase enzyme production on solid agar medium using bromocresol purple for developing the clear zone around colonies, and characterized due to its antagonistic effect against mycelia growth of pathogenic fungi. The nucleotide sequences (submitted and retrieved from NCBI) of all ten Trichoderma species are analyzed through TrichOKEY 2 program for their validation post molecular identification. This has confirmed the selected sequences as specific strains of Trichoderma species. [ABSTRACT FROM AUTHOR]