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Acceleration of the onset of collagen-induced arthritis by a deficiency of platelet endothelial cell adhesion molecule 1.
- Source :
- Arthritis & Rheumatism; Nov2003, Vol. 48 Issue 11, p3280-3290, 11p
- Publication Year :
- 2003
-
Abstract
- Platelet endothelial cell adhesion molecule 1 (PECAM-1; CD31) is a member of the immunoglobulin superfamily that is expressed in platelets, leukocytes, and endothelial cells. PECAM-1 has been shown to play a role in transendothelial migration of leukocytes and contains immunoreceptor tyrosine-based inhibitory motifs in its cytoplasmic tail and inhibits cellular responses. We examined the role of PECAM-1 in the development of collagen-induced arthritis (CIA). CIA was induced in PECAM-1deficient DBA/1 mice. The incidence of arthritis and the arthritis index were examined. Antitype II collagen (anti-CII) antibody levels and interferon-γ (IFNγ) production by lymph node cells and spleen cells were determined. Lymphocytes from arthritic PECAM-1deficient and wild-type mice were labeled with dye, transferred to arthritic PECAM-1<superscript>+/-</superscript> mice, and cell migration to inflamed joints was examined. PECAM-1deficient mice showed accelerated onset of arthritis and increased severity only during the early phase. Anti-CII antibody levels were also increased during the early phase. IFNγ production by lymph node cells and spleen cells from PECAM-1deficient mice in response to CII was higher than that in wild-type mice. Lymphocytes from arthritic PECAM-1deficient mice showed accelerated migration to inflamed joints, but not lymph nodes or spleen. The development of anti-CII antibodyinduced arthritis was similar in PECAM-1deficient and wild-type mice. These results indicate that PECAM-1 negatively regulates humoral and cell-mediated immune responses and lymphocyte migration into joints and, consequently, the development of CIA. In addition, the role of PECAM-1 in the transendothelial migration of leukocytes appears to be redundant in this model. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00043591
- Volume :
- 48
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- Arthritis & Rheumatism
- Publication Type :
- Academic Journal
- Accession number :
- 11433610
- Full Text :
- https://doi.org/10.1002/art.11268