Effect of Dendrobium moniliforme on Melanogenic Protein Expression in B16F10 Melanoma Cells.

TheDendrobium moniliformeextract (DME) was successively partitioned usingn-hexane, CH2Cl2, EtOAc, BuOH, and water. The results indicate that theDendrobium moniliformefraction extracted using CH2Cl2(DMC) was an effective inhibitor of melanogenesis in murine melanoma cells (B16F10). To elucidate the mechanism of the effect of DME on melanogenesis, we performed western blotting of the melanogenic proteins. DME inhibited tyrosinase and, tyrosinase-related protein-1 (TRP-1) and TRP-2 expressions. Futher, we confirmed that DME decreased the protein level of melanocyte-specific isoform of microphthalmia-associated transcription factor (MITF) proteins, which decreased tyrosinase and related genes in B16F10 melanoma cells. On the basis of the results, we suggest thatD. moniliformeis effective against hyperpigmentation disorders and that it be considered a possible anti-melanogenic agent in topical application. [ABSTRACT FROM PUBLISHER]