Back to Search
Start Over
Biotechnological production of acetoin, a bio-based platform chemical, from a lignocellulosic resource by metabolically engineered Enterobacter cloacae.
- Source :
- Green Chemistry; 3/21/2016, Vol. 18 Issue 6, p1560-1570, 11p
- Publication Year :
- 2016
-
Abstract
- Acetoin (AC) is regarded as one of the top potential sugar-derived chemical building blocks that can be used as food additives, precursors in chemical synthesis, and plant growth promoting molecules. In this study, a low-cost lignocellulosic resource of pretreated corn stover was used as a carbon source to produce AC. After redirecting the metabolic flux, fine tuning reducing power, and eliminating carbon catabolite repression in Enterobacter cloacae SDM, a systematically engineered strain SDM 53 was constructed, which is able to utilize glucose and xylose efficiently and simultaneously. Using fed-batch fermentation of SDM 53, 45.6 g L<superscript>−1</superscript> AC was produced at a rate of 1.52 g L<superscript>−1</superscript> h<superscript>−1</superscript> using the lignocellulosic hydrolysate. Biotechnological synthesis of AC has various advantages such as being sustainable and environment-friendly. With its desirable properties, the engineered strain SDM 53 may be a potential choice for the industrial production of AC. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14639262
- Volume :
- 18
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Green Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 113842844
- Full Text :
- https://doi.org/10.1039/c5gc01638j