Library synthesis of cardiomyogenesis inducing compounds using an efficient two-step-one-flow process.

Within this work we telescoped a batch laboratory scale synthesis towards a two-step-one-flow process to synthesize cardiomyogenesis inducing compounds (4,6-diaminopyrimidines) in continuous manner. Special attention was put on a quick and robust screening protocol and subsequent UHPLC analysis. Finally, the robustness of the method was proven by the multi-gram synthesis of VUT-MK142 using a laboratory scale continuous flow reactor, enabling to deliver enough material for extensive biological testing. Graphical abstract: [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]