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Microbial yield from physiotherapy assisted sputum production in respiratory outpatients.
- Source :
- BMC Pulmonary Medicine; 2/2/2016, Vol. 16, p1-8, 8p, 3 Diagrams, 3 Charts
- Publication Year :
- 2016
-
Abstract
- <bold>Background: </bold>Sputum is a key diagnostic sample for those with chronic chest conditions including chronic and allergic aspergillus-related disease, but often not obtained in clinic. The objective of this study was to evaluate physiotherapeutic interventions to obtain sputum from those not able to spontaneously produce and the subsequent microbiological result.<bold>Methods: </bold>Sputum samples were collected by physiotherapists from patients attending routine outpatient clinics managing their aspergillus-related diseases who were unable to spontaneously produce. Active Cycle of Breathing Techniques (ACBT) technique was applied first, for 10 min, followed by hypertonic saline induction using a Pari LC plus or Pari Sprint nebuliser, if necessary and deemed safe to do so. Samples processed in the laboratory using standard microbiological techniques for bacterial and fungal culture with the addition of Aspergillus real-time PCR.<bold>Results: </bold>Samples were procured from 353 of 364 (97%) patients, 231 (65%) by ACBT and 119 (34%) with administration of hypertonic saline. Three of 125 (2.4%) patients had significant bronchospasm during sputum induction. Sixteen patients' sputum tested positive for Aspergillus culture, contrasting with 82 whose Aspergillus PCR was positive, 59 with a strong signal. PCR improved detection of Aspergillus by 350%. Sputum from 124 (34%) patients cultured other potentially pathogenic organisms which justified specific therapy.<bold>Conclusions: </bold>Physiotherapeutic interventions safely and effectively procured sputum from patients unable to spontaneously produce. The method for sputum induction was well-tolerated and time-efficient, with important microbiological results. [ABSTRACT FROM AUTHOR]
- Subjects :
- SPUTUM
PHYSICAL therapy research
ASPERGILLUS
PNEUMOCYSTIS pneumonia
MICROBIOLOGY
MYCOSES
ASPERGILLOSIS diagnosis
ASTHMA diagnosis
RESPIRATORY allergy diagnosis
RESPIRATORY disease diagnosis
SPUTUM microbiology
ALLERGIES
OUTPATIENT medical care
ASPERGILLOSIS
ASTHMA
HAEMOPHILUS diseases
HAEMOPHILUS influenzae
PHYSICAL therapy
POLYMERASE chain reaction
PSEUDOMONAS
PSEUDOMONAS diseases
PULMONARY aspergillosis
RESPIRATORY allergy
RESPIRATORY diseases
RESPIRATORY therapy
HYPERTONIC saline solutions
DISEASE complications
DIAGNOSIS
Subjects
Details
- Language :
- English
- ISSN :
- 14712466
- Volume :
- 16
- Database :
- Complementary Index
- Journal :
- BMC Pulmonary Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 112762137
- Full Text :
- https://doi.org/10.1186/s12890-016-0188-2