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A Dual Tracer 18F-FCH/18F-FDG PET Imaging of an Orthotopic Brain Tumor Xenograft Model.
- Source :
- PLoS ONE; 2/4/2016, Vol. 11 Issue 2, p1-13, 13p
- Publication Year :
- 2016
-
Abstract
- Early diagnosis of low grade glioma has been a challenge to clinicians. Positron Emission Tomography (PET) using <superscript>18</superscript>F-FDG as a radio-tracer has limited utility in this area because of the high background in normal brain tissue. Other radiotracers such as <superscript>18</superscript>F-Fluorocholine (<superscript>18</superscript>F-FCH) could provide better contrast between tumor and normal brain tissue but with high incidence of false positives. In this study, the potential application of a dual tracer <superscript>18</superscript>F-FCH/<superscript>18</superscript>F-FDG-PET is investigated in order to improve the sensitivity of PET imaging for low grade glioma diagnosis based on a mouse orthotopic xenograft model. BALB/c nude mice with and without orthotopic glioma xenografts from U87 MG-luc2 glioma cell line are used for the study. The animals are subjected to <superscript>18</superscript>F-FCH and <superscript>18</superscript>F-FDG PET imaging, and images acquired from two separate scans are superimposed for analysis. The <superscript>18</superscript>F-FCH counts are subtracted from the merged images to identify the tumor. Micro-CT, bioluminescence imaging (BLI), histology and measurement of the tumor diameter are also conducted for comparison. Results show that there is a significant contrast in <superscript>18</superscript>F-FCH uptake between tumor and normal brain tissue (2.65 ± 0.98), but with a high false positive rate of 28.6%. The difficulty of identifying the tumor by <superscript>18</superscript>F-FDG only is also proved in this study. All the tumors can be detected based on the dual tracer technique of <superscript>18</superscript>F-FCH/ <superscript>18</superscript>F-FDG-PET imaging in this study, while the false-positive caused by <superscript>18</superscript>F-FCH can be eliminated. Dual tracer <superscript>18</superscript>F-FCH/<superscript>18</superscript>F-FDG PET imaging has the potential to improve the visualization of low grade glioma. <superscript>18</superscript>F-FCH delineates tumor areas and the tumor can be identified by subtracting the <superscript>18</superscript>F-FCH counts. The sensitivity was over 95%. Further studies are required to evaluate the possibility of applying this technique in clinical trials. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 112749645
- Full Text :
- https://doi.org/10.1371/journal.pone.0148123