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Fabrication of a tunable hydrogel membrane for constructing indirect cell coculture system.

Authors :
Song, Yizhe
Zheng, Guoshuang
Zhang, Demeng
Lv, Yan
Li, Na
Wang, Xiuli
Yu, Weiting
Ma, Xiaojun
Source :
Journal of Applied Polymer Science; 3/20/2016, Vol. 133 Issue 12, p1-N.PAG, 8p
Publication Year :
2016

Abstract

In this study, an improved indirect cell coculture system was constructed by using a polyelectrolyte complex membrane generated by alginate (A) and chitosan (C). Methodologies of characterizing thickness and permeability of flat AC membrane were first established due to the importance of these two parameters in determining intercellular distance and degree of contact between cocultured cells. Compared to reaction time, both alginate concentration and molecular weight (M<subscript>w</subscript>) of chitosan play more dominant roles in determining the membrane thickness and diffusion coefficients. Moreover, cells in the alginate gel and on the AC membrane could maintain high cell viability. Thus, an improved indirect cell coculture system constructed by flat AC membrane was fabricated and characterized, which provides a robust tool to study the effect of intercellular distance and degree of contact between cocultured cells on cell-cell interactions. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00218995
Volume :
133
Issue :
12
Database :
Complementary Index
Journal :
Journal of Applied Polymer Science
Publication Type :
Academic Journal
Accession number :
112037800
Full Text :
https://doi.org/10.1002/app.43100