Impaired aquaporins expression in the gastrointestinal tract of rat after mercury exposure.

The main route of exposure to mercury in humans is through the diet. Consequently, the gastrointestinal mucosa is exposed to the mercurial forms, where they cause intestinal fluid accumulation, mucosal injuries and diarrhea. The relationship between inorganic mercury (HgCl₂) and methylmercury (CH₃HgCl) exposure and water movement in the gastrointestinal tract is still unexplored. The leading role of aquaporins (AQPs) in the rapid bidirectionalmovement of fluid in the gastrointestinal tract of mammals is well established. The present study evaluates the effect of HgCl₂ and CH₃HgCl exposure on AQP expression in different portions of the gastrointestinal tract of rats treated by gavage (5 mg kg^-1 of mercury species, single dose, 4 days). The results show thatmercury species reducemRNA and protein levels of AQPs in different parts of the gastrointestinal tract. In the stomach, treated rats show a significant reduction of expression of AQP3 (80-90% for mRNA and 50% for protein) and AQP4 (95-99% for mRNA and 20-40%for protein). In the small and large intestine, treated rats experience a significant reduction of AQP3 and AQP7 expression. Protein contents of both AQPs are reduced in similar proportions in jejunum(AQP3: 40-50%; AQP7: 45-50%) and colon (AQP3: 35-40%; AQP7: 45-60%), regardless of the treatment. Our results indicate that some AQPs are downregulated in the rat gastrointestinal tract by mercury exposure, suggesting a possible role of AQPs in the development ofmercury gastrointestinal symptoms. [ABSTRACT FROM AUTHOR]