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High glucose induces renal tubular epithelial injury via Sirt1/NF-kappaB/microR-29/Keap1 signal pathway.

Authors :
Ling Zhou
De-yu Xu
Wen-gang Sha
Lei Shen
Guo-yuan Lu
Xia Yin
Ming-jun Wang
Zhou, Ling
Xu, De-Yu
Sha, Wen-Gang
Shen, Lei
Lu, Guo-Yuan
Yin, Xia
Wang, Ming-Jun
Source :
Journal of Translational Medicine; 11/9/2015, Vol. 13, p1-12, 12p
Publication Year :
2015

Abstract

<bold>Objective: </bold>Diabetic nephropathy (DN) is a serious complication that commonly confronted by diabetic patients. A common theory for the pathogenesis of this renal dysfunction in diabetes is cell injury, inflammation as well as oxidative stress. In this content, the detailed molecular mechanism underlying high glucose induced renal tubular epithelial injury was elaborated. <bold>Methods: </bold>An in vivo rat model of diabetes by injecting streptozotocin (STZ) and an in vitro high glucose incubated renal tubular epithelial cell (HK-2) model were used. Expression levels of Keap1, nuclear Nrf2 and p65 were determined by western blotting. Level of microR-29 (miR-29) was assessed using quantitative RT-PCR. Combination of p65 and miR-29 promotor was assessed using chromatin immunoprecipitation. Keap1 3'-UTR activity was detected using luciferase reporter gene assay. Cell viability was determined using MTT assay. <bold>Results: </bold>In diabetic rat, miR-29 was downregulated and its expression is negatively correlated with both of serum creatinine and creatinine clearance. In high glucose incubated HK-2 cell, deacetylases activity of Sirt1 was attenuated that leads to decreased activity of nuclear factor kappa B (NF-κB). NF-κB was demonstrated to regulate miR-29 expression by directly binding to its promotor. The data of luciferase assay showed that miR-29 directly targets to Keap1 mRNA. While high glucose induced down regulation of miR-29 contributed to enhancement of Keap1 expression that finally reduced Nrf2 content by ubiquitinating Nrf2. Additionally, overexpression of miR-29 effectively relieved high glucose-reduced cell viability. <bold>Conclusion: </bold>High glucose induces renal tubular epithelial injury via Sirt1/NF-κB/microR-29/Keap1 signal pathway. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14795876
Volume :
13
Database :
Complementary Index
Journal :
Journal of Translational Medicine
Publication Type :
Academic Journal
Accession number :
110910010
Full Text :
https://doi.org/10.1186/s12967-015-0710-y