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Discrimination of human semen specimens by NMR data, sperm parameters, and statistical analysis.

Discrimination of human semen specimens by NMR data, sperm parameters, and statistical analysis.

Authors :
Bonechi, Claudia
Collodel, Giulia
Donati, Alessandro
Martini, Silvia
Moretti, Elena
Rossi, Claudio
Source :
Systems Biology in Reproductive Medicine; Dec2015, Vol. 61 Issue 6, p353-359, 7p
Publication Year :
2015

Abstract

Human seminal fluid is a complex mixture of secretions originated from epididymis and the male accessory sex glands. It contains a variety of both inorganic and organic components, among which proteins are a major part of the high molecular-mass substances. In this study, 83 human seminal plasma samples were analyzed using a combined Nuclear Magnetic Resonance (NMR) Spectroscopy and Principal Component Analysis (PCA) approach to discriminate patients in relation to semen characteristics and/or conditions affecting the fertility status. Results showed a discrimination between patients with leukocytospermia and with the concomitant presence of varicocele/ex varicocele and leukocytospermia. Patients with testicular cancer, necrozoospermia, and azoospermia were separated from the other patient clusters. In addition, a differentiation of semen quality was also possible. This study represents to first use of sperm parameters together with NMR data as variables in the PCA analysis. Furthermore, this methodology allows the identification of the metabolites which play the most important role in identifying differences among human seminal plasma samples. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
19396368
Volume :
61
Issue :
6
Database :
Complementary Index
Journal :
Systems Biology in Reproductive Medicine
Publication Type :
Academic Journal
Accession number :
110900762
Full Text :
https://doi.org/10.3109/19396368.2015.1054003