Effects of acute systemic administration of TiO 2 , ZnO, SiO 2 , and Ag nanoparticles on hemodynamics, hemostasis and leukocyte recruitment.

It has been suggested that engineered nanomaterials (ENM), once arrived in the circulation, may affect the cardiovascular system. The aim of thisin vivostudy was to screen major cardiovascular effects of acute systemic administration of a panel of five nanomaterials, TiO2anatase (NM-101), TiO2rutile (NM-104), ZnO (NM-110), SiO2(NM-200) and Ag (NM-300). Mice were anesthetized and the ENM were injected at a dose of 1 mg/kg via a catheter placed in the left femoral artery. Hemodynamic parameters were determined by invasive measurement of blood pressure and non-invasive measurement of heart rate. Ten minutes after injection of the ENM, the formation of light/dye-induced thrombi was assessed in the cremasteric microcirculation by intravital microscopy. In addition, the numbers of rolling, firmly adherent and transmigrated leukocytes were recorded in postcapillary cremasteric venules over a time period of 120 min after injection of ENM by intravital microscopy. The systemic administration of a single dose of the ENM tested did not dramatically alter hemodynamic parameters or affect early steps of leukocyte recruitment. However, the presence of circulating TiO2anatase, but not of TiO2rutile, SiO2, ZnO or Ag nanoparticles, significantly accelerated thrombus formation in the murine microcirculation. Moreover, TiO2anatase but not TiO2rutile nanoparticles increased murine platelet aggregationin vitro. Taken together, only one of the five systemically administered ENM, TiO2anatase, affected hemostasis, whereas none of the ENM tested in this screening study dramatically modulated hemodynamic parameters or early steps of leukocyte recruitment. [ABSTRACT FROM PUBLISHER]