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Hyperbranched lysine−arginine copolymer for gene delivery.
- Source :
- Journal of Biomaterials Science -- Polymer Edition; Nov2015, Vol. 26 Issue 16, p1163-1177, 15p, 2 Black and White Photographs, 2 Diagrams, 5 Graphs
- Publication Year :
- 2015
-
Abstract
- Based on the reactivity of amine groups and carboxyl groups ofL-lysine andL-arginine, thermal polymerization of these two natural amino acids results in hyperbranched lysine–arginine copolymers (P-lys-argX, whereXrefers to the relevant molar ratio of arginine to lysine). Hyperbranched polylysine (P-lys) and two derivatives (P-lys-arg0.10and P-lys-arg0.20) have been prepared. The arginine-rich hyperbranched polymers can interact with plasmid DNA to form nano-sized particles. The polyplexes were physicochemically analyzed by agarose gel electrophoresis, dynamic light scattering, and zeta potential measurements. Furthermore, their transfection efficiency was assessed, employing COS-7, 293T, and HeLa cell lines. It was found that P-lys showed poorly in its ability of condensation with DNA and transfection efficiency. On the other hand, arginine-rich products resulted to significant enhancement of its transfection efficiency, which is dependent on the content of arginine in the polymers, and the cell line used. P-lys-arg0.20exhibited better transfection efficiency under all the condition studied. Besides, P-lys-arg0.20showed lower toxicity in COS-7 cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- LYSINE
ARGININE
COPOLYMERS
GENE delivery techniques
CARBOXYL group
AMINES
Subjects
Details
- Language :
- English
- ISSN :
- 09205063
- Volume :
- 26
- Issue :
- 16
- Database :
- Complementary Index
- Journal :
- Journal of Biomaterials Science -- Polymer Edition
- Publication Type :
- Academic Journal
- Accession number :
- 109927071
- Full Text :
- https://doi.org/10.1080/09205063.2015.1080482