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Expression of a rice glutaredoxin in aleurone layers of developing and mature seeds: subcellular localization and possible functions in antioxidant defense.

Expression of a rice glutaredoxin in aleurone layers of developing and mature seeds: subcellular localization and possible functions in antioxidant defense.

Authors :
Morita, Shigeto
Yamashita, Yuki
Fujiki, Masayoshi
Todaka, Rie
Nishikawa, Yuri
Hosoki, Ayaka
Yabe, Chisato
Nakamura, Jun'ichi
Kawamura, Kazuyoshi
Suwastika, I.
Sato, Masa
Masumura, Takehiro
Ogihara, Yasunari
Tanaka, Kunisuke
Satoh, Shigeru
Source :
Planta: An International Journal of Plant Biology; Nov2015, Vol. 242 Issue 5, p1195-1206, 12p
Publication Year :
2015

Abstract

Main Conclusion: A rice glutaredoxin isoform (OsGrxC2;2) with antioxidant capacity is expressed abundantly in seed tissues and is localized to storage vacuoles in aleurone layers in developing and mature seeds. Seed tissues undergo drastic water loss at the late stage of seed development, and thus need to tolerate oxidative injuries associated with desiccation. We previously found a rice glutaredoxin isoform, OsGrxC2; 2, as a gene expressed abundantly in developing seeds. Since glutaredoxin is involved in antioxidant defense, in the present study we investigated the subcellular localization and expression profile of OsGrxC2;2 and whether OsGrxC2;2 has a role in the defense against reactive oxygen species. Western blotting and immunohistochemistry revealed that the OsGrxC2;2 protein accumulated at a high level in the embryo and aleurone layers of developing and mature seeds. The OsGrxC2;2 in developing seeds was particularly localized to aleurone grains, which are storage organelles derived from vacuoles. Overexpression of OsGrxC2;2 resulted in an enhanced tolerance to menadione in yeast and methyl viologen in green leaves of transgenic rice plants. These results suggest that OsGrxC2;2 participates in the defense against oxidative stress in developing and mature seeds. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00320935
Volume :
242
Issue :
5
Database :
Complementary Index
Journal :
Planta: An International Journal of Plant Biology
Publication Type :
Academic Journal
Accession number :
109420162
Full Text :
https://doi.org/10.1007/s00425-015-2354-9