Back to Search
Start Over
Regulation of Angiogenic Functions by Angiopoietins through Calcium-Dependent Signaling Pathways.
- Source :
- BioMed Research International; 6/4/2015, Vol. 2015, p1-14, 14p
- Publication Year :
- 2015
-
Abstract
- Angiopoietins are vascular factors essential for blood vessel assembly and correct organization and maturation. This study describes a novel calcium-dependent machinery activated through Angiopoietin-1/2-Tie receptor system in HUVECs monolayer. Both cytokines were found to elicit intracellular calcium mobilization. Targeting intracellular Ca<superscript>2+</superscript> signaling, antagonizing IP<subscript>3</subscript> with 2-APB or cADPR with 8Br-cADPR, was found to modulate in vitro angiogenic responses to Angiopoietins in a specific way. 2-APB and 8Br-cADPR impaired the phosphorylation of AKT and FAK induced by Ang-1 and Ang-2. On the other hand, phosphorylation of ERK1/2 and p38, as well as cell proliferation, was not affected by either inhibitor. The ability of ECs to migrate following Angs stimulation, evaluated by “scratch assay,” was reduced by either 2-APB or 8Br-cADPR following Ang-2 stimulation and only slightly affected by 2-APB in cells stimulated with Ang-1. These results identify a novel calcium-dependent machinery involved in the complex interplay regulating angiogenic processes showing that IP<subscript>3</subscript>- and cADPR-induced Ca<superscript>2+</superscript> release specifically regulates distinct Angs-mediated angiogenic steps. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 23146133
- Volume :
- 2015
- Database :
- Complementary Index
- Journal :
- BioMed Research International
- Publication Type :
- Academic Journal
- Accession number :
- 109274491
- Full Text :
- https://doi.org/10.1155/2015/965271