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Enhanced Shewanella biofilm promotes bioelectricity generation.
- Source :
- Biotechnology & Bioengineering; Oct2015, Vol. 112 Issue 10, p2051-2059, 9p
- Publication Year :
- 2015
-
Abstract
- ABSTRACT Electroactive biofilms play essential roles in determining the power output of microbial fuel cells (MFCs). To engineer the electroacitve biofilm formation of Shewanella oneidensis MR-1, a model exoelectrogen, we herein heterologously overexpressed a c-di-GMP biosynthesis gene ydeH in S. oneidensis MR-1, constructing a mutant strain in which the expression of ydeH is under the control of IPTG-inducible promoter, and a strain in which ydeH is under the control of a constitutive promoter. Such engineered Shewanella strains had significantly enhanced biofilm formation and bioelectricity generation. The MFCs inoculated with these engineered strains accomplished a maximum power density of 167.6 ± 3.6 mW/m<superscript>2</superscript>, which was ∼ 2.8 times of that achieved by the wild-type MR-1 (61.0 ± 1.9 mW/m<superscript>2</superscript>). In addition, the engineered strains in the bioelectrochemical system at poised potential of 0.2 V vs. saturated calomel electrode (SCE) generated a stable current density of 1100 mA/m<superscript>2</superscript>, ∼ 3.4 times of that by wild-type MR-1 (320 mA/m<superscript>2</superscript>). Biotechnol. Bioeng. 2015;112: 2051-2059. © 2015 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00063592
- Volume :
- 112
- Issue :
- 10
- Database :
- Complementary Index
- Journal :
- Biotechnology & Bioengineering
- Publication Type :
- Academic Journal
- Accession number :
- 109078837
- Full Text :
- https://doi.org/10.1002/bit.25624