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Treatment with hESC-Derived Myocardial Precursors Improves Cardiac Function after a Myocardial Infarction.

Authors :
Ye, Jianqin
Gaur, Meenakshi
Zhang, Yan
Sievers, Richard E.
Woods, Brandon J.
Aurigui, Julian
Bernstein, Harold S.
Yeghiazarians, Yerem
Source :
PLoS ONE; 7/31/2015, Vol. 10 Issue 7, p1-12, 12p
Publication Year :
2015

Abstract

Background: We previously reported the generation of a reporter line of human embryonic stem cells (hESCs) with enhanced green fluorescent protein (eGFP) expression driven by the α-myosin heavy chain (αMHC) promoter. The GFP<superscript>+</superscript>/αMHC<superscript>+</superscript> cells derived from this cell line behave as multipotent, human myocardial precursors (hMPs) in vitro. In this study, we evaluated the therapeutic effects of GFP<superscript>+</superscript>/αMHC<superscript>+</superscript> cells isolated from the reporter line in a mouse model of myocardial infarction (MI). Methods: MI was generated in immunodeficient mice. hMPs were injected into murine infarcted hearts under ultrasound guidance at 3 days post-MI. Human fetal skin fibroblasts (hFFs) were injected as control. Cardiac function was evaluated by echocardiography. Infarct size, angiogenesis, apoptosis, cell fate, and teratoma formation were analyzed by immunohistochemical staining. Results: Compared with control, hMPs resulted in improvement of cardiac function post-MI with smaller infarct size, induced endogenous angiogenesis, and reduced apoptosis of host cardiomyocytes at the peri-infarct zone at 28 days post-MI. Conclusion: Intramyocardial injection of hMPs improved cardiac function post-MI. The engraftment rate of these cells in the myocardium post-MI was low, suggesting that the majority of effect occurs via paracrine mechanisms. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
19326203
Volume :
10
Issue :
7
Database :
Complementary Index
Journal :
PLoS ONE
Publication Type :
Academic Journal
Accession number :
108665199
Full Text :
https://doi.org/10.1371/journal.pone.0131123