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A Newcomer′s Guide to Peptide Crystallography.
- Source :
- Israel Journal of Chemistry; Jun2015, Vol. 55 Issue 6/7, p698-710, 13p
- Publication Year :
- 2015
-
Abstract
- Herein we provide a guide for adapting the tools developed for protein X-ray crystallography to study the structures and supramolecular assembly of peptides. Peptide crystallography involves selecting a suitable peptide, crystallizing the peptide, collecting X-ray diffraction data, processing the diffraction data, determining the crystallographic phases and generating an electron density map, building and refining models, and depositing the crystallographic structure in the Protein Data Bank (PDB). Advances in technology make this process easy for a newcomer to adopt. This paper describes techniques for determining the X-ray crystallographic structures of peptides: incorporation of amino acids containing heavy atoms for crystallographic phase determination, commercially available kits to crystallize peptides, modern techniques for X-ray crystallographic data collection, and free user-friendly software for data processing and producing a crystallographic structure. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00212148
- Volume :
- 55
- Issue :
- 6/7
- Database :
- Complementary Index
- Journal :
- Israel Journal of Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 108353805
- Full Text :
- https://doi.org/10.1002/ijch.201400179