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Tracking the acetate threshold using DO-transient control during medium and high cell density cultivation of recombinant Escherichia coli in complex media.
- Source :
- Biotechnology & Bioengineering; 2003, Vol. 84 Issue 3, p314, 10p
- Publication Year :
- 2003
-
Abstract
- DO-transient nutrient controllers use the dissolved oxygen signal to attempt acetate threshold tracking during fed-batch cultivation of recombinant E. coli. Here we apply DO-transient control to the production of Jembrana disease virus protein in complex Super Luria medium and compare performance against a high-limit pH-stat controller. For induction at medium cell density (harvest between 31 and 32.5 g dcw L) a total productivity of 0.27 g L h was achieved as compared to 0.24 g L h with the high-limit pH-stat. For induction at high cell density (harvest at 60 g dcw L), decreased productivity (0.12 g L h) was attributed to the effect of acetate accumulation on recombinant protein formation and a concomitant lowering of the critical growth rate. Our results suggest that complex media provides a difficult environment for the application of acetate threshold tracking DO-transient control because of difficulties in re-oxidizing acetate, and apparent localized production of acetate below the production threshold (as detected by the DO-transient controller as SPOUR<subscript>crit</subscript>). Configuring the DO-transient controller to avoid aggressive threshold probing is suggested as a means to improve performance and reduce acetate accumulation in complex media. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 84: 314323, 2003. [ABSTRACT FROM AUTHOR]
- Subjects :
- ESCHERICHIA coli
OXYGEN
PROTEINS
ACETATES
MOLECULAR biology
Subjects
Details
- Language :
- English
- ISSN :
- 00063592
- Volume :
- 84
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Biotechnology & Bioengineering
- Publication Type :
- Academic Journal
- Accession number :
- 10816383
- Full Text :
- https://doi.org/10.1002/bit.10772