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Quantitative polymerase chain reaction to predict occurrence of symptomatic cytomegalovirus infection and assess response to ganciclovir therapy in renal transplant recipients.

Authors :
Roberts TC
Brennan DC
Buller RS
Gaudreault-Keener M
Schnitzler MA
Sternhell KE
Garlock KA
Singer GG
Storch GA
Roberts, T C
Brennan, D C
Buller, R S
Gaudreault-Keener, M
Schnitzler, M A
Sternhell, K E
Garlock, K A
Singer, G G
Storch, G A
Source :
Journal of Infectious Diseases; 1998, Vol. 178 Issue 3, p626-635, 10p
Publication Year :
1998

Abstract

Cytomegalovirus (CMV) DNA levels were measured by quantitative-competitive polymerase chain reaction (PCR) in weekly leukocyte samples from 50 renal transplant recipients, including 23 with symptomatic and 27 with asymptomatic CMV infection. Peak and week 4 CMV DNA levels were higher in symptomatic subjects (P = .07 and .02, respectively). In a logistic regression model, the logarithm of the week 4 level independently predicted symptomatic infection (odds ratio, 1.78 for a 1 log10 increase; 95% confidence interval, 1.14-2.78; P = .01). All subjects whose week 4 level exceeded 1000 copies/100,000 leukocytes developed symptoms. In subjects with adequate samples for analysis, CMV levels declined exponentially with ganciclovir treatment, with an average half-life of 3.3 days. Levels exceeding 10,000 copies were associated with prolonged time to clearing of CMV DNA. Potential clinical applications of quantitative CMV PCR include predicting occurrence of symptomatic first episodes after transplantation and individualizing duration of antiviral therapy. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00221899
Volume :
178
Issue :
3
Database :
Complementary Index
Journal :
Journal of Infectious Diseases
Publication Type :
Academic Journal
Accession number :
106994489
Full Text :
https://doi.org/10.1086/515383