Science. Factor V Leiden and prothrombin G20210A mutations among healthy Indians in Malaysia.

Background: Factor V Leiden (FVL) (G1691A) and prothrombin gene (G20210A) mutations are the 2 most common inherited forms of thrombophilia. The prevalence of these 2 mutations is known to show a distinct world distribution and is most prevalent among Caucasians. Previous studies report that these mutations are rare among other populations including Malays and Chinese. The aim of this study was to determine the frequency of FVL and prothrombin gene mutations among healthy Indians in Malaysia without a family history of venous thrombosis. Materials and Methods: DNA from 71 apparently healthy Indians was analyzed for the detection of FVL and prothrombin gene mutations, using PCR-RFLP with MnlI and allele-specific PCR respectively. Results: Out of the 71 samples analyzed, 4 were heterozygous (5.6%) for FVL mutation while no homozygous FVL mutation was detected. Prothrombin gene mutation was totally absent among our subjects. Conclusion: Factor V Leiden mutation in this region probably gives different clinical outcomes, suggesting the need for future studies on FVL and environmental interactions. [ABSTRACT FROM AUTHOR]