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Basal HIF-1α expression levels are not predictive for radiosensitivity of human cancer cell lines.
- Source :
- Strahlentherapie und Onkologie; Apr2012, Vol. 188 Issue 4, p353-358, 6p
- Publication Year :
- 2012
-
Abstract
- <bold>Background and Purpose: </bold>High levels of hypoxia inducible factor (HIF)-1α in tumors are reported to be associated with tumor progression and resistance to therapy. To examine the impact of HIF-1α on radioresistance under normoxia, the sensitivity towards irradiation was measured in human tumor cell lines that differ significantly in their basal HIF-1α levels.<bold>Material and Methods: </bold>HIF-1α levels were quantified in lysates of H1339, EPLC-272H, A549, SAS, XF354, FaDu, BHY, and CX- tumor cell lines by ELISA. Protein levels of HIF-1α, HIF-2α, carbonic anhydrase IX (CA IX), and GAPDH were assessed by Western blot analysis. Knock-down experiments were performed using HIF-1α siRNA. Clonogenic survival after irradiation was determined by the colony forming assay.<bold>Results: </bold>According to their basal HIF-1α status, the tumor cell lines were divided into low (SAS, XF354, FaDu, A549, CX-), intermediate (EPLC-272H, BHY), and high (H1339) HIF-1α expressors. The functionality of the high basal HIF-1α expression in H1339 cells was proven by reduced CA IX expression after knocking-down HIF-1α. Linear regression analysis revealed no correlation between basal HIF-1α levels and the survival fraction at either 2 or 4 Gy in all tumor cell lines investigated.<bold>Conclusion: </bold>Our data suggest that basal HIF-1α levels in human tumor cell lines do not predict their radiosensitivity under normoxia. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01797158
- Volume :
- 188
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Strahlentherapie und Onkologie
- Publication Type :
- Academic Journal
- Accession number :
- 104443458
- Full Text :
- https://doi.org/10.1007/s00066-011-0051-6