Qualitative analyzing method based on real-time fluorescence quantitative PCR for identifying rabbit hair.

To establish a rapid, specific and sensitive method for the detection of rabbit hair, and provide an effective detecting evidence for rabbit hair, a pair of primers and probe corresponding to the rabbit COX I gene for real time fluorescence quantitative PCR were designed, similar sequences were searched by Blast method, and the excellent primers and probe no cross reaction with common livestock were selected. DNA from common animal fiber such as duck down, sheep wool, cashmere, yak hair, alpaca fiber was used for specific test. The extracted rabbit DNA were serially diluted for real time PCR amplification, the sensitivity of method was tested. Three times repeated experiments with primer, verify its repeatability. The primers and probe according to the rabbit cox I gene could only amplify rabbit hair DNA, but not other reference animal fiber DNA. Real time fluorescence quantitative PCR can rapidly and accurately qualitative tests the rabbit hair contamination in fiber products with high specificity, repeatability and sensitivity. [ABSTRACT FROM AUTHOR]