Methanotrophy within the water column of a large meromictic tropical lake (Lake Kivu, East Africa).

The permanently stratified Lake Kivu is one of the largest freshwater reservoirs of dissolved methane (CH₄) on Earth. Yet CH₄ emissions from its surface to the atmosphere have been estimated to be 2 orders of magnitude lower than the CH₄ upward flux to the mixed layer, suggesting that microbial CH₄ oxidation is an important process within the water column. A combination of natural abundance stable carbon isotope analysis (δ¹³C) of several carbon pools and ¹³CH₄-labelling experiments was carried out during the rainy and dry season to quantify (i) the contribution of CH₄ -derived carbon to the biomass, (ii) methanotrophic bacterial production (MBP), and (iii) methanotrophic bacterial growth efficiency (MBGE), defined as the ratio between MBP and gross CH₄ oxidation. We also investigated the distribution and the δ¹³ C of specific phospholipid fatty acids (PLFAs), used as biomarkers for aerobic methanotrophs. Maximal MBP rates were measured in the oxycline, suggesting that CH₄ oxidation was mainly driven by oxic processes. Moreover, our data revealed that methanotrophic organisms in the water column oxidized most of the upward flux of CH₄, and that a significant amount of CH₄ -derived carbon was incorporated into the microbial biomass in the oxycline. The MBGE was variable (2--50 %) and negatively related to CH₄ :O₂ molar ratios. Thus, a comparatively smaller fraction of CH₄ -derived carbon was incorporated into the cellular biomass in deeper waters, at the bottom of the oxycline where oxygen was scarce. The aerobic methanotrophic community was clearly dominated by type I methanotrophs and no evidence was found for an active involvement of type II methanotrophs in CH₄ oxidation in Lake Kivu, based on fatty acids analyses. Vertically integrated over the water column, the MBP was equivalent to 16--60% of the average phytoplankton particulate primary production. This relatively high magnitude of MBP, and the substantial contribution of CH₄ - derived carbon to the overall biomass in the oxycline, suggest that methanotrophic bacteria could potentially sustain a significant fraction of the pelagic food web in the deep, meromictic Lake Kivu. [ABSTRACT FROM AUTHOR]