Effect of JGYS on Ovariectomy-induced Rat High-turnover Osteoporosis.

Objective To study the effects of JGYS on bone weight, bone mineral density, and bone calcium, and to evaluate the therapeutic effects on ovariectomy (OVX) -induced rat osteoporosis and its dose -- effect relationship. Methods Female Sprague-dawly (SD) rats were randomly divided into an ovariectomized (OVX) group and a sham operation group. The rats in OVX group were ovariectomized. However, for the rats in sham group, only a part of fat near both ovaries were removed under the same anesthesia and surgical approach. Bone mineral density, mechanical properties, and serum biochemical parameters were examined 90 d after OVX to characterize the experimental animal model. After osteoporotic rat model establishment, rats in OVX group were divided into model group, diethylstilbestrol group, 300 mg/kg, 600 mg/kg, 1 200 mg/kg JGYS groups, and 500 mg/kg Xianlingubao capsule, ten rats in each group. All the above drugs were administered orally for 90 d. Bone mineral density and serum biochemical parameters were examined respectively by dual-energy X-ray absorptiometry analysis and enzyme-linked immunoassay. Results (1) Effect on the uterus index: compared with model group, 1200 mg/kg JGYS could obviously reduce the weight of rats, and increase the uterus index; 600 mg/kg JGYS reduced weight and increased the uterus index; 300 mg/kg JGYS had no significant effect on rat weight and the uterus index; the effect on the uterus index of 600 mg/kg JGYS was similar to Xianlingubao capsule, but significantly weaker than diethylstilbestrol. (2) Effect on bone metabolism index: (1) the effect on serum ALP, TRAP: compared with the model group, 600 and 1200 mg/kg JGYS could significantly decrease the activity of ALP and TRAP, 300 mg/kg JGYS had no obvious effect on the activity of ALP and TRAP. The function of 600 mg/kg JGYS was similar to Xianlingubao capsule and diethylstilbestrol. (2) the effect on the blood calcium and blood phosphorus: compared with OVX group, three doses of JGYS showed no significant effect either on blood calcium or on blood phosphorus levels. (3) Effect on bone mass: (1) the effect on femoral bone mineral density and bone mineral content: compared with the model group, 600-1200 mg/kg JGYS groups could significantly increase femoral bone mineral density and bone mineral content;300 mg/kg JGYS showed no significant effect. The effect of increasing femoral bone mineral density and bone mineral content for 600 mg/kg JGYS was same as 500 mg/kg Xianlingubao capsule and 0.03 mg/kg diethylstilbestrol. (2) the effect of vertebral bone mineral density and bone mineral content : three doses of Yishenjiangu tablet obviously increased vertebral bone mineral density and bone mineral content. The effect on increasing the vertebral bone mineral density and bone mineral content was similar to 500 mg/kg Xianlingubao capsule; the 1200 mg/kg JGYS tablet's function for increasing vertebral bone mineral density was better than Xianlingubao capsule. (3) the effect on wet, dry, and ash weight of bone and bone calcium content: compared with the model group, 600 and 1200 mg/kg JGYS significantly increased femoral and vertebral bone calcium content, and increased the content of wet, dry weight and ash weight; 300 mg/kg JGYS had no significant effect on the former index, but increase the calcium and bone of vertebral bone. The function of 600 mg/kg JGYS was similar to 500 mg/kg Xianlingubao capsule, but weaker than 0.03 mg/kg diethylstilbestrol. Conclusion JGYS can modulate bone metabolism and increase bone mass, and shows a therapeutic effect on OVX-induced rat osteoporosis in a dose -- effect manner. [ABSTRACT FROM AUTHOR]