Cloning and expression characteristics of one ω-3 cis15 fatty acid dehydrogenase gene AhFAD3A in Arachis hypogaea L.

The content of α-linolenic acid varied in different germplasm resources and different seed developmental stages in peanut oil. Oneα-linolenic acid synthesis related gene ω-3Δ¹⁵ fatty acid dehydrogenase gene, AhFAD3A was cloned from cotyledonary tissues of peanut germinating seeds using RACE and bioinformatics analysis. Its expression patterns were analyzed in different tissues and different period of seed developmental stages by the fluorescence quantitative real-time PCR method between two α- linolenic acid synthesis genes, AhFAD3A and AhFAD8. The results showed that: AhFAD3A gene was mainly expressed in leaf, root and young seed tissue during the period of flowering, and relatively low expression was found in the other developmental stages. This result verified that the gene expression was positively correlated to the formation of α-linolenic acid in seed. AhFAD8A gene was expressed in the green tissue during the whole growth stage of peanut, not detected in non optical tissues. These results laid a foundation for further research in determining the function of AhFAD3A gene, its expression regulation and its relationship with the formation ofα-linolenic acid in seed. [ABSTRACT FROM AUTHOR]