Cloning and expression analysis of chalcone synthetase and isomerase in kenaf (Hibiscus cannabinus L.).

[Objective] The present study was conducted to clone chalcone synthetase (CHS) and chalcone isomerase (CHI) genes in kenaf, and construct CHS plant expression vector in order to lay the foundation for further researches of their functions in kenaf ferility and stress resistance. [Method] The DNA and cDNA of kenaf male sterility (P3A) and maintainer line (P3B) anthers were amplified after extracting of DNA. The CHS and CHI genes were cloned by using homologous cloning method based on kenaf transcriptome data from high-throughput sequencing. By using carrier recombination technology, the plant expression vector of CHS gene was constructed. [Result] It showed that, the cDNA full-length sequence of CHS was 1236 bp in length, contained an open reading frame (ORF) of 1170 bp, and encoded 389 amino acids. The DNA full-length sequence of CHS gene was 1329 bp in length, and contained two exons and one intron. The cDNA full-length sequence of CHI was 724 bp which contained an open reading frame (ORF) of 630 bp and encoded protein with 209 amino acids. The length of DNA sequence in CHI gene was 1087 bp in length which contained four exons and three introns. [Conclusion] The sequences of CHS and CHI genes was successfully cloned, and the constructed plant expression vector pBI121-CHS of CHS gene could be used for its functional researches. [ABSTRACT FROM AUTHOR]