Synthesis, characterization and biological evaluation of Tc-labeled Mitomycin C.

Mitomycin-C is used for the treatment of different types of tumours. In present work, a reliable method was developed for radiolabeling of Mitomycin-C with Tc for diagnostic purpose. Tc-Mitomycin-C was obtained with radiochemical yield of 100 % by adding 200 µg Mitomycin-C, 1 mL (15 mCi) of pertechnetate in 25 μg SnCl·2HO at pH 7. Labeling efficiency was determined by paper chromatography and ITLC. The charge on Tc-Mitomycin-C was determined by electrophoresis technique. HPLC analyses were performed for the determination of purity of Mitomycin-C and radiochemical purity of labeled complex. Evaluation of Tc-Mitomycin-C, in vitro stability, biodistribution and scintigraphic images in normal mice were performed. [ABSTRACT FROM AUTHOR]