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Stabilizing immobilized cellulase by ionic liquids for saccharification of cellulose solutions in 1-butyl-3-methylimidazolium chloride.
- Source :
- Green Chemistry; Jun2011, Vol. 13 Issue 6, p1406-1410, 5p
- Publication Year :
- 2011
-
Abstract
- A new approach to improve the cellulase stability against 1-butyl-3-methylimidazolium chloride ([Bmim][Cl]), based on coating of immobilized enzyme particles with hydrophobic ILs, is proposed. The stability of commercial cellulase (Celluclast<superscript>®</superscript>), immobilized onto a polymeric support (Amberlite XAD4), was first studied in ten different ionic liquids (ILs) at 50 and 80 °C. Hydrophobic ILs clearly enhanced the enzyme thermal stability. Butyltrimethyl-ammonium bis(trifluoromethylsulfonyl)imide ([N<subscript>1114</subscript>][NTf<subscript>2</subscript>]) enhances half-life time of the immobilized enzyme at 50 °C up to 4 times, while [Bmim][Cl] behaved as a powerful enzyme deactivating agent. Thus, the stability of cellulase in hydrophobic IL/[Bmim][Cl] mixtures was greatly improved with respect to [Bmim][Cl] alone. A stabilized cellulase derivative obtained by coating immobilized enzyme particles with [N<subscript>1114</subscript>][NTf<subscript>2</subscript>], has been then successfully used for the saccharification of dissolved cellulose in [Bmim][Cl] (i.e. up to 50% hydrolysis in 24 h) at 50 °C and 1.5 w/v water content. [ABSTRACT FROM AUTHOR]
- Subjects :
- CELLULASE
IONIC liquids
CELLULOSE
IMIDAZOLES
THERMAL stability
IMMOBILIZED enzymes
Subjects
Details
- Language :
- English
- ISSN :
- 14639262
- Volume :
- 13
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Green Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 100891630
- Full Text :
- https://doi.org/10.1039/c1gc15294g