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Sensitivity and dose dependency of radiation-induced injury in hematopoietic stem/progenitor cells in mice.

Authors :
Guo, Chang-Ying
Luo, Lan
Urata, Yoshishige
Goto, Shinji
Huang, Wen-Jing
Takamura, Syu
Hayashi, Fumiko
Doi, Hanako
Kitajima, Yuriko
Ono, Yusuke
Ogi, Tomoo
Li, Tao-Sheng
Source :
Scientific Reports; 1/30/2015, p8055, 1p
Publication Year :
2015

Abstract

We evaluated the sensitivity and dose dependency of radiation-induced injury in hematopoietic stem/progenitor cells. Adult C57BL/6 mice were daily exposed to 0, 2, 10, 50, and 250 mGy γ-ray for 1 month in succession, respectively. The damage of hematopoietic stem/progenitor cells in bone marrow were investigated within 2 hours (acute phase) or at 3 months (chronic phase) after the last exposure. Daily exposure to over 10 mGy γ-ray significantly decreased the number and colony-forming capacity of hematopoietic stem/progenitor cells at acute phase, and did not completely recover at chronic phase with 250 mGy exposure. Interestingly, the daily exposure to 10 or 50 mGy γ-ray decreased the formation of mixed types of colonies at chronic phase, but the total number of colonies was comparable to control. Immunostaining analysis showed that the formation of 53BP1 foci in c-kit<superscript>+</superscript> stem/progenitor cells was significantly increased with daily exposure to 50 and 250 mGy at acute phase, and 250 mGy at chronic phase. Many genes involved in toxicity responses were up- or down-regulated with the exposures to all doses. Our data have clearly shown the sensitivity and dose dependency of radiation-induced injury in hematopoietic stem/progenitor cells of mice with daily exposures to 2 ~ 250 mGy γ-ray. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20452322
Database :
Complementary Index
Journal :
Scientific Reports
Publication Type :
Academic Journal
Accession number :
100760806
Full Text :
https://doi.org/10.1038/srep08055