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Low copy number detection of human bocavirus DNA in bronchoalveolar lavage of asymptomatic adult patients.
Low copy number detection of human bocavirus DNA in bronchoalveolar lavage of asymptomatic adult patients.
- Source :
- Future Virology; Aug2014, Vol. 9 Issue 8, p715-720, 6p
- Publication Year :
- 2014
-
Abstract
- Aim: The aim of this study was to investigate the percentage of such asymptomatic human bocavirus (HBoV) DNA-shedding patients with a sensitive commercial method. Materials & methods: Bronchoalveolar lavage fluids were analyzed with the xTag<superscript>®</superscript> respiratory viral panel (RVP) assay (Luminex, The Netherlands), the RespiFinder<superscript>®</superscript> SMART assay (PathoFinder, Maastricht, The Netherlands) and an in-house real-time PCR protocol. Results: While replacing the Luminex xTag RVP assay with the RespiFinder SMART assay for routine diagnostics, surprisingly, more than 50% of bronchoalveolar lavage fluid samples from asymptomatic patients previously tested with negative Luminex xTag RVP tested positive for HBoV. Conclusion: As the RespiFinder SMART assay has a higher sensitivity in the present study, this supports the theory that HBoV persists and is capable of shedding low copy numbers of viral genomes at very low numbers, even in asymptomatic people. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 17460794
- Volume :
- 9
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Future Virology
- Publication Type :
- Academic Journal
- Accession number :
- 100428221
- Full Text :
- https://doi.org/10.2217/fvl.14.59