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Low copy number detection of human bocavirus DNA in bronchoalveolar lavage of asymptomatic adult patients.

Low copy number detection of human bocavirus DNA in bronchoalveolar lavage of asymptomatic adult patients.

Authors :
Kaur, Jaspreet
Schildgen, Verena
Tillmann, Ramona-Liza
Hardt, Anna-Lena
Lüsebrink, Jessica
Windisch, Wolfram
Schildgen, Oliver
Source :
Future Virology; Aug2014, Vol. 9 Issue 8, p715-720, 6p
Publication Year :
2014

Abstract

Aim: The aim of this study was to investigate the percentage of such asymptomatic human bocavirus (HBoV) DNA-shedding patients with a sensitive commercial method. Materials & methods: Bronchoalveolar lavage fluids were analyzed with the xTag<superscript>®</superscript> respiratory viral panel (RVP) assay (Luminex, The Netherlands), the RespiFinder<superscript>®</superscript> SMART assay (PathoFinder, Maastricht, The Netherlands) and an in-house real-time PCR protocol. Results: While replacing the Luminex xTag RVP assay with the RespiFinder SMART assay for routine diagnostics, surprisingly, more than 50% of bronchoalveolar lavage fluid samples from asymptomatic patients previously tested with negative Luminex xTag RVP tested positive for HBoV. Conclusion: As the RespiFinder SMART assay has a higher sensitivity in the present study, this supports the theory that HBoV persists and is capable of shedding low copy numbers of viral genomes at very low numbers, even in asymptomatic people. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
17460794
Volume :
9
Issue :
8
Database :
Complementary Index
Journal :
Future Virology
Publication Type :
Academic Journal
Accession number :
100428221
Full Text :
https://doi.org/10.2217/fvl.14.59