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Bosentan protects the spinal cord from ischemia reperfusion injury in rats through vascular endothelial growth factor receptors.
- Source :
- Spinal Cord; Jan2015, Vol. 53 Issue 1, p19-23, 5p, 3 Graphs
- Publication Year :
- 2015
-
Abstract
- Study design:Experimental study.Objectives:To investigate whether Bosentan, an endothelin-A/-B dual receptor antagonist, could protect neurons after spinal cord ischemia reperfusion (SCIR) injury in rats and its underlying signaling pathway.Setting:Department of Neurosurgery, the Second Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi Province, China.Methods:Sprague-Dawley rats were randomly divided into two groups, saline group (IRS, n=48) and Bosentan group (IRB, 5 mg kg<superscript>−1</superscript>, n=48). After ischemia for 1 h with occlusion of the infrarenal aorta, spinal cord were reperfused for 6h, 12h, 24h, 3d, 5d, and 7d separately. Enzyme-linked immunosorbent assay was used to detect vascular endothelial growth factor (VEGF) in serum. Immunohistochemistry was performed to detect protein expression of VEGF, VEGF receptor 1 (FLT-1) and VEGF receptor 2 (FLK-1). Gene expressions of VEGF and its receptors were evaluated using the quantitative reverse transcription polymerase chain reaction.Results:Compared with the IRS group, gene and protein expressions of VEGF, FLT-1 and FLK-1 were significantly increased (P<0.05), so was the concentration of VEGF in plasma (P<0.05). FLK-1 was expressed on spinal cord neurons. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13624393
- Volume :
- 53
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Spinal Cord
- Publication Type :
- Academic Journal
- Accession number :
- 100317616
- Full Text :
- https://doi.org/10.1038/sc.2014.147