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Biological stability and activity of siRNA in ionic liquids.
- Source :
- Chemical Communications; 2014, Vol. 50 Issue 88, p13457-13460, 4p
- Publication Year :
- 2014
-
Abstract
- We report substantially enhanced biological stability of siRNA in a hydrated ionic liquid (IL) based on buffered choline dihydrogen phosphate (CDP). The results show that hydrated CDP IL substantially prolongs the shelf-life of siRNA for up to three months in the presence of RNase A. Remarkably, siRNA stored in the IL remained active and eGFP siRNA exhibited clear gene knockdown effects in eGFP-expressing He La cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- SMALL interfering RNA
IONIC liquids
GENE expression
BIOPOLYMERS
FLOW cytometry
Subjects
Details
- Language :
- English
- ISSN :
- 13597345
- Volume :
- 50
- Issue :
- 88
- Database :
- Complementary Index
- Journal :
- Chemical Communications
- Publication Type :
- Academic Journal
- Accession number :
- 100188938
- Full Text :
- https://doi.org/10.1039/c4cc05086j